LIFA荧光寿命系统附件

    简 述:
    LIFA是适用于荧光寿命显微成像(FLIM)。该设备可以利用频域技术在任何宽场显微荧光成像系统获得寿命变化相关图像。系统包含TRiCAM M可调制型像增强CCD相机,可调制光源,调制控制器,定制电脑以及LI-FLIM软件。像增强相机包含像增强器,CCD相机,可以获得*高增益和采集速率。
    品 牌:
    荷兰 Lambert Instruments
    产品型号:
    • LIFA
    LIFA荧光寿命系统附件
    LIFA是适用于荧光寿命显微成像(FLIM)。该设备可以利用频域技术在任何宽场显微荧光成像系统获得寿命变化相关图像。系统包含TRiCAM M可调制型像增强CCD相机,可调制光源,调制控制器,定制电脑以及LI-FLIM软件。像增强相机包含像增强器,CCD相机,可以获得*高增益和采集速率。
    LIFA荧光寿命系统附件关键特点:
    精准的寿命成分:0-300ns的荧光寿命,精度可达30ps RMS
    系统技术参数:
    Lifetime range0 - 300 ns
    Lifetime resolutionless than 100 ps (single pixel)
    Speed2 lifetime images per second (full frame)
    Spatial resolution26 lp/mm (Gen II); 19 lp/mm (Gen III)
    Sensitivitysingle photon
    Intrascene dynamic range12 bit
    Camera input format1 inch
    Effective pixel size10.3 μm square (12,9 for lens coupled CCD)
    控制器参数:控制器可以经USB线LI-FLIM软件控制
    Signal shapeSinusoidal
    Frequency range1 MHz – 120 MHz
    Frequency adjustment resolution1 kHz
    Frequency deviation100 ppm max.
    Harmonics suppressionBetter than 25 dB
    Output 1
    Type a: MultiLED
    AC signal level
    DC current adjust
    Capable of driving lightsource type a, optionally b and c
    Adjustable in 250 steps
    0 – 1000 mA, 12 bit resolution
    Optional lightsource outputs:
    Type b: Modulated laser diode
    AC signal level
    DC level adjust
    Type c: AOM for CW laser
    AC signal level
    Frequency
    Output 2
    AC signal level
    AC signal after amplification
    0.00 – 1.00 V peak-peak
    0.00 – 1.00 V
    0.00 – 1.00 V peak-peak
    Half the frequency of output 2
    To image intensifier
    Adjustable from –50 to 0 dBm
    15 Vpp nom.
    External trigger inputLVTTL (phase shift on falling edge or rising edge)
    Impedance50 Ω
    Phase adjustment range0 - 360° in 1° steps
    Phase deviation< 0.01°
    Cathode voltage DCAdjustable from –150 V to +15 V
    MCP voltage (gain) DCAdjustable from 450 – 1000 V max.
    Anode voltage DCFixed 6 kV
    多LED光源
    Peak wavelengths (nm), approximately405 (CFP), 445 (cerulean, CFP), 470 (GFP), 535 (TRITC), 630 (CY5), others on request
    Number of mounted LEDs3 (standard), or 4 (maximum)
    Modulation frequencyup to at least 80 MHz
    LEDs switching time200ms
    CouplingCompatible with existing Hg and Xe microscope adapters
    Lifetime driftless than 30 ps*
    Optional Temperature stabilization for
    long-term (hours) lifetime stability
    Active thermoelectric water cooling/heating system
    Control temperature range5 – 45º C
    Temperature stability (constant load,
    constant ambient temp)
    ± 0.1º C
    Dimensions (l x w x h)346 x 109 x 183 mm
    * Measured using stable fluorescent material with single lifetime component of 3.2 ns
    多激光光源
    Wavelengths (nm)375 (BFP), 405 (CFP), 445 (cerulean, CFP), 488 (GFP), 515 (YFP, Venus), 647 (CY5), others on request
    Number of laser linesStandard: up to 3 or up to 6; Extreme: up to 4. All combined into one optical fiber output
    Output power (mW)20 mW-300 mW, depending on the wavelength
    Modulation frequencyUp to 120 MHz (standard)
    Input signalDigital: 0...1 V / 50 Ω
    Control interfaceRS232
    CouplingKineFLEX system, with a single-mode polarization maintaining fiber and either FC-APC, FC-PC or FC-P8 output connector
    Power stabilityStandard: 2%*, Extreme: 0.5%*
    Lifetime driftStandard: less than 50 ps*, Extreme: less than 20-30 ps*
    Dimensions (l x w x h)19" rack housing. MultiLASER-3: 480 x 484 x 88 mm; MultiLASER-4 and 6: 610 x 484 x 132 mm
    Options• Multi-channel excitation
    • Two single-mode optical fiber outputs
    • Custom fiber outputs on special request
    * Measured using stable fluorescent material with single lifetime component of 3.2 ns
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